Table 2.

Percentage of morphology abnormalities. Hos-positive and viable spermatozoa (means ± SE) after post-thaw and selected sperm

	Post-thaw sperm (%)					Selected sperm (%)
Bulls (n)	1(2)	2(2)	3(2)	4(2)	P	1(2)	2(2)	3(2)	4(2)	P
Proximal cytoplasmic droplets	0.00 ± 0.00	0.20 ± 0.20	0.00 ± 0.00	0.00 ± 0.00	0.392	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	1.000
Pyriformheads	0.23 ± 0.23	0.44 ± 0.44	0.39 ± 0.39	1.80 ± 0.99	0.344	2.51 ± 0.32	2.81 ± 0.73	1.25 ± 0.87	4.16 ± 2.61	0.608
Strongly folded/coiled tails	0.23 ± 0.23	0.44 ± 0.44	0.39 ± 0.39	1.80 ± 0.99	0.344	0.62 ± 0.26	0.92 ± 0.09	1.21 ± 0.07	2.17 ± 1.01	0.129
Midpiecedefects	0.45 ± 0.45	0.88 ± 0.88	0.97 ± 0.19	1.77 ± 1.46	0.809	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	1.000
Maldeveloped	0.00 ± 0.00	0.44 ± 0.44	0.00 ± 0.00	0.00 ± 0.00	0.392	0.00 ± 0.00	0.67 ± 0.16	0.00 ± 0.00	0.19 ± 0.19	0.116
Craters	0.22 ± 0.22	4.24 ± 1.45	0.00 ± 0.00	1.22 ± 0.72	0.161	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	1.000
Primaryabnormalities	1.13 ± 0.20	6.66 ± 0.56	2.18 ± 0.17	5.36 ± 0.52	0.083	3.13 ± 0.06	4.39 ± 0.66	2.47 ± 0.94	6.52 ± 3.44	0.367
Distal droplets	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	1.000	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	0.00 ± 0.00	1.000
Tailless heads	1.14 ± 0.21	1.50 ± 0.27	1.77 ± 0.99	1.27 ± 0.34	0.908	3.89 ± 2.14	0.26 ± 0.26	1.41 ± 0.13	0.79 ± 0.02	0.083
Simple bent/terminal coiled tails	1.82 ± 0.89	1.26 ± 0.38	2.53 ± 0.96	1.52 ± 0.90	0.682	1.94 ± 0.19	2.9 ± 1.66	3.47 ± 0.8	1.55 ± 1.15	0.682
Small/giantheads	3.45 ± 0.75	6.22 ± 1.30	6.44 ± 0.24	4.40 ± 1.17	0.212	5.87 ± 0.17	7.47 ± 7.47	3.38 ± 0.83	11.54 ± 1.98	0.446
Abaxial implantations	0.24 ± 0.24	0.87 ± 0.46	1.95 ± 0.38	0.20 ± 0.20	0.148	0.18 ± 0.18	1.38 ± 0.14	1.26 ± 0.88	0.59 ± 0.19	0.198
Abnormal acrosomes	12.54 ± 2.58	10.32 ± 1.48	16.46 ± 1.68	13.15 ± 0.27	0.244	7.75 ± 1.83	26.32 ± 3.05	15.98 ± 0.57	14.81 ±2.31	0.112
Secondary abnormalities	19.17 ± 2.46	20.17 ± 0.18	29.13 ± 1.80	20.54 ± 0.00	0.193	25.89 ± 4.62	47.11 ± 7.17	30.41 ± 3.17	42.32 ± 1.26	0.139
Total abnormalities	20.30 ± 2.25	26.83 ± 0.73	31.31 ± 1.63	25.90 ± 0.52	0.104	29.02 ± 4.68	51.5 ± 6.51	32.87 ± 4.1	48.85 ± 4.7	0.129
Hos-positivespermatozoa	36.68 ± 5.68	43.41 ± 2.77	39.38 ± 10.2	52.08 ± 6.25	0.539	64.51 ± 1.88	72.97 ± 0.48	62.71 ± 1.17	61.40 ± 4.32	0.212
Viability	53.49 ± 1.22	40.77 ± 4.72	30.83 ± 8.76	35.86 ± 1.94	0.212	82.37 ± 4.47	73.58 ± 6.77	73.62 ± 6.53	67.62 ± 1.08	0.446

n; Number of straws from one freezing batch.