Table 2. Environmental DNA sample results from bromeliads in three mountain ranges across Trinidad, West Indies.

Results are ordered by tree and by total bromeliad diameter in meters, small (S < 0.33 m), medium (M 0.33–0.66 m), or large (L > 0.66 m). Total eDNA copies is the copy number estimated for the whole sample.

Mountain Peak	Tree ID	Sample ID	Volume (ml)	Bromeliad Size	Frog Visual	Positive replicates	Total eDNA copies
Cerro del Aripo	1	A11	150	L	P. auratus	3/3	357878
	1	A12	50	L	P. auratus	3/3	54459
	1	A08	75	L		2/3	45
	1	A09	100	L	Tadpole	3/3	56984
	1	A10	100	L		3/3	579
	1	A06	50	M	F. fitzgeraldi	0/3	0
	1	A07	50	M		3/3	503878
	2	A01	100	M		3/3	948
	2	A02	100	M		0/3	0
	2	A03	100	M		0/3	0
	2	A04	100	M		3/3	3244
	2	A05	100	M		3/3	6833
El Tucuche	1	ET11	150	L		3/3	255
	1	ET08	235	L		0/3	0
	1	ET06	150	M		3/3	288
	1	ET07	30	S		2/3	35
	1	ET09	120	S		3/3	597
	1	ET10	150	S	F. fitzgeraldi	0/3	0
	1	ET12	175	S		3/3	185
	2	ET03	175	L		3/3	211615
	2	ET02	125	M		3/3	4069
	2	ET01	125	M		3/3	177
	2	ET04	30	S		2/6	23
	3	ET05	100	M		3/3	10972
	Ground	ET13	100	M		2/6	152
Chaguaramal	1	CHA03	200	L		3/3	168
	1	CHA04	250	L		0/3	0
	1	CHA01	150	M		3/3	1562
	1	CHA02	100	M	Unknown Frog	3/6	23