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A mechanistic investigation of AST by digital PCR (dPCR) after beta lactam exposure and non-denaturing DNA extraction using shearing to disrupt macromolecular assemblies; error bars for qPCR are 2.8 S.D. (see SI), N=3; error bars are 98%C.I. for dPCR (see SI), N=2. Significant (p-value ≤ 0.02) and nonsignificant p-values for susceptible isolates quantified using dPCR are denoted with a green check and red × respectively (see SI).
