Table 2. Primers used for detecting both copy number changes and expression changes in 7 candidate genes.

Analysis	Gene	Primers	Length of products (bp)
Copy number detection	CREB3L4	F: 5′-TTCCGTTTGTGGACCCTCAG-3′	296
		R: 5′-CCTCACCTGTCCCCTCGATA-3′
	TRIP13	F: 5′-CCCCAGCACTTCGGTTCA-3′	116
		R: 5′-GCCCTTTCTCCCGCCTTT-3′
	CCNE2	F: 5′-CATGGTCGGATTAACTCACACG-3′	336
		R: 5′-CCTGCATTCTGTCCCACCTTA-3′
	AHRR	F: 5′-CAGACAGGCAGGAATGAACA-3′	116
		R: 5′-TAGGAAGGAAGGGAAGG-3′
	KLF10	F: 5′-TTGTCATCCAAATGACACACAGA-3′	256
		R: 5′-GTGCCTCTCTCCCATGAACG-3′
	MYC	F: 5′-AGAGTTTCATCTGCGACCCG-3′	259
		R: 5′-AGAGGGTAGGGGAAGACCAC-3′
	NKD2	F: 5′-CCTAAACTGGGCATCTGTGG-3′	119
		R: 5′-CTCTCTGGCTCCTGCTGACT-3′
	GAPDH	F: 5′-CCACCACACTGAATCTCCCC-3′	262
		R: 5′-CGAAGCAAGCAAGGCTGTTT-3′
Gene expression detection	CREB3L4	F: 5′-CTGCCCTGTCAAACCCTGTT-3′	142
		R: 5′-GCTTGTTACGGATTTTCCTCCT-3′
	TRIP13	F: 5′-CTGGAGGAAGAGACAGAAAACATAA-3′	134
		R: 5′-GTTGTCATCACATAATCGAGGAGAT-3′
	CCNE2	F: 5′-GGAACCACAGATGAGGTCCAT-3′	237
		R: 5′-CCATCAGTGACGTAAGCAAACT-3′
	AHRR	F: 5′-GCGGACGGTTCTTCCTAATC-3′	116
		R: 5′-GCAGTTTCCTGTGTCTTCTC-3′
	KLF10	F: 5′-CTTCCGGGAACACCTGATTTT-3′	161
		R: 5′-GCAATGTGAGGTTTGGCAGTATC-3′
	MYC	F: 5′-GGCTCCTGGCAAAAGGTCA-3′	119
		R: 5′-CTGCGTAGTTGTGCTGATGT-3′
	NKD2	F: 5′-CCGACAGCAAACAGCAACT-3′	156
		R: 5′-AGCCTTAGAGCCAGGAAACA-3′
	GAPDH	F: 5′-TGTTGCCATCAATGACCCCTT-3′	202
		R: 5′-CTCCACGACGTACTCAGCG-3′