Table 3. (a) Primer list used to clone greater amberjack leptin; (b) Comparable sequence from closely related species for primer design; (c) Primers for greater amberjack real-time PCR.

(a)	Target gene	Sequence
	Seriola Lep F1	GAAATCAAAAGTGAAATGGATGG
	Seriola Lep F3	CCAGGTCCCTCCTGGCCTGAC
	Seriola Lep R3	TTGACCTGRGWGACYCCRTY
(b)	Target gene	Species—Accession number
	Leptin	Atlantic bluefin tuna—HQ288053
(c)	Target gene	Real time-PCR Primers
	leptin FOR	CCGTTAAGGGTGTCAGAGA
	leptin REV	TTCCAGGTCCCTGTTGGTC
	β-actin FOR	CCCTGTCCTGCTCACAGAGG
	β-actin REV	CAAGTCCAGACGCAGGATGG