Table 2.

Tissue infectious assay and histopathologic diagnoses of adult AG129 mice infected with ZIKV.

Days post-infection	Tissue infectious assay on indicator Vero 76 cells				Histopathologic lesions^a	ZIKV immunohistochemistry^b

	Kidney pos./total^c	Liver pos./total	Testis pos./total	Brain pos./total	Brain pos./total	Testis pos./total	Epididymis pos./total
Sham	0/3	0/3	0/3	0/3	0/3	0/3	0/3
3	0/4	N/A	N/A	N/A	0/3	0/2	0/2
5	0/4	N/A	N/A	N/A	0/4	2/2	0/2
7	3/4	2/4	0/2	2/4	0/4	2/2	2/2
9	0/4	1/4	2/2	3/4	4/4^d	0/1	0/1
11	2/4	0/4	2/2	2/4	4/4^d	0/2	0/2
13	N/A	N/A	N/A	N/A	2/2^d	1/1	1/1

N/A: Not available.

Histopathologic analysis of kidney, spleen, liver and testis/uterus were also processed and did not have any histopathologic lesions as assessed by a board-certified veterinary pathologist.

Various tissues, including testis and attached epididymis, brain, spleen, liver and kidney were processed. In addition to the results above, spleen also stained positive for ZIKV immunoreactivity after 5 dpi in 2 animals.

Samples that stained positive for ZIKV foci per total number of samples assayed.

Meningoencephalitis was the primary lesion observed in brain sections of ZIKV-infected mice.