Fig 3. Reduction in macrophage IL-10 production by zinc is ZIP8-dependent.

ZIP8 protein levels (A) are reduced following LPS (100 ng/mL) exposure in MDMs transfected with siRNA targeting ZIP8 (anti-ZIP8) compared to scramble control (SC) siRNA. Densitometric analysis of Panel 3A reveals that ZIP8 protein levels are reduced by over 60% following knockdown. Zinc-dependent reduction in macrophage (B) IL-10 production but not (C) TNFα (D) IL-8 and (E) IL-6 production is ZIP8-dependent as determined by ELISA of cell free supernatants following ZIP8 knockdown or scramble control and 6 or 24 h co-stimulation with LPS (100 ng/mL) and ZnSO₄ (18 μM). Panel A is representative of 3 experiments. Panels B, C, D and E are cumulative data from 5 different donors (mean ± SEM; ^#p < 0.05).