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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Curr Biol. 2016 Apr 14;26(9):1148–1158. doi: 10.1016/j.cub.2016.03.036

Figure 1. cep-1 and him-5 Cooperate to Maintain Chromosome Stability during Meiosis.

Figure 1

(A) Structural features of CEP-1 protein showing the N-terminal domain (N-term), DNA-binding domain (DBD), oligomerization domain (OD), and C-terminal sterile alpha motif (SAM). The gk138 allele is a 1,630-bp in-frame deletion spanning the DBD that is predicted to generate a truncated protein retaining the OD and SAM domains. The lg12501 allele is a 1,231-bp frameshift deletion that removes part of the DBD and OD.

(B) Quantification of lethality among F1 progeny of cep-1 and him-5 single mutants and cep-1;him-5 double mutants. Data are represented as mean ± SEM; n, broods counted. Statistical comparisons between him-5 single mutants and cep-1;him-5 double mutants were performed using the two-tailed Mann-Whitney test; 95% CI. ***p < 0.0001, **p = 0.002. See also Table S1.

(C) Representative confocal images of DAPI-stained diakinesis chromosomes of young adult animals (day 1 or ~24 hr post-L4 stage) of the indicated genotypes. The scale bar represents 5 µm. See also Figure S1.

(D) Quantification of DAPI-stained bodies in diakinesis oocytes of young adult animals (day 1) of the indicated genotypes. n, number of oocytes.