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. Author manuscript; available in PMC: 2017 Jan 6.
Published in final edited form as: Curr Biol. 2016 Apr 14;26(9):1148–1158. doi: 10.1016/j.cub.2016.03.036

Figure 2. Synthetic Lethality with him-5 Is Independent of Apoptosis but Involves the DDR Checkpoint.

Figure 2

(A) Simplified schematic of the DNA damage checkpoint pathway in the C. elegans germline.

(B) Quantification of total lethality among F1 progeny of ced-3(n717) and him-5(e1490) single mutants and ced-3(n717);him-5(e1490) double mutants. Data are represented as mean ± SEM; n, broods counted. Statistical comparisons between combined lethality of ced-3(n717) and him-5(e1490) single mutants and ced-3(n717);him-5(e1490) double mutants were performed using the two-tailed Mann-Whitney test; 95% CI. p = 0.3508. See also Figure S2A.

(C) Quantification of apoptotic corpses in cep-1(lg12501) and him-5(e1490) single and cep-1(lg12501);him-5(e1490) double mutants under physiological conditions. Data are represented as mean ± SEM; n, germlines counted. Statistical comparisons between wild-type and him-5 single mutants were performed using the two-tailed Mann-Whitney test; 95% CI. p = 0.1704.

(D) Quantification of total lethality among F1 progeny of hus-1(op244), him-5(e1490), and atm-1(gk186) single mutants and hus-1(op244);him-5(e1490) and atm-1(gk186);him-5(e1490) double mutants. Data are represented as mean ± SEM; n, broods counted. Statistical comparisons between genotypes were performed using the two-tailed Mann-Whitney test; 95% CI. him-5(e1490) versus hus-1(op244); him-5(e1490), ***p < 0.0001;him-5(1490) versus atm-1(gk186);him-5(e1490), *p = 0.0159.

(E) Representative confocal image of DAPI-stained diakinesis chromosomes of young adult (Day 1) hus-1(op244);him-5(e1490) mutants. Scale bar represents 5 µm. See also Figure S2B.

(F) Representative confocal image of DAPI-stained diakinesis chromsomes of young adult (Day 1) atm-1(gk186);him-5(e1490) mutants. Scale bar represents 5 µm. See also Figure S2C.