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. 2017 Jan 5;12(1):e0169225. doi: 10.1371/journal.pone.0169225

Fig 2. Enrichment of GR, CTCF, acetyl-H3K27, and RNA polymerase II at the ANGPTL4 gene locus in cells treated with Dex.

Fig 2

(A) Enrichment of glucocorticoid receptor (GR) in cells treated with Dex. As shown in Fig 1B, HepG2 cells were treated with Dex for 24 h. ChIP-qPCR analysis was performed using an anti-GR antibody and an anti-rabbit IgG (control), followed by quantitative PCR using specific primers for each AG site and the control (NC). (B–D) Enrichment of CTCF, acetyl-H3K27 (H3K27ac), and active RNA polymerase II (Pol2 ser5-P) in cells treated with Dex. ChIP-qPCR analyses were performed using an anti-CTCF antibody and an anti-rabbit IgG (control) (B), anti-H3K27ac (C), and anti-Pol2 ser5-P (D), followed by quantitative PCR using specific primers for each indicated site. Relative enrichment of the control (NC) site was normalized to 1 (D). Asterisks indicate statistically significance between control (Dex 0 h) and Dex-treated cells at each time point. *P < 0.05, **P < 0.01, ***P < 0.005.