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. 2017 Jan 5;12(1):e0169225. doi: 10.1371/journal.pone.0169225

Fig 6. Model of ANGPTL4 gene regulation and higher-order chromatin in cells treated with Dex.

Fig 6

In its basal state, ANGPTL4 is not activated in the absence of Dex-bound GR within the preexisting higher-order chromatin structure. When ANGPTL4 is transcriptionally active (Dex 3 h), liganded GR binds to AG sites and enhances interactions between enhancer, promoter, and CTCF sites to selectively induce ANGPTL4 but not the neighboring KANK3, RAB11B-AS, and RAB11B genes. In the silenced state (Dex 24 h), the positions of enhancer, promoter, and CTCF sites are restored, leading to down-regulation of ANGPTL4 transcription. CTCF is required for proper induction and subsequent silencing of the ANGPTL4 transcription.