. Author manuscript; available in PMC: 2017 Mar 15.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Feb 9;1015-1016:34–41. doi: 10.1016/j.jchromb.2016.02.012

Table 2.

Extraction recovery, intra-day and inter-day precision, and matrix effects for irinotecan, SN-38 and SN-38 glucuronide in plasma using MRM method (n=6)

Compound	Conc. (nM)	Extraction recovery Average ± SD (%)	Intra-day		Inter-day		Matrix effect Average ± SD (%)

			Accuracy (bias, %)	Precision (CV, %)	Accuracy (bias, %)	Precision (CV, %)
	40	106.9 ± 4.45	104.34	13.19	103.48	6.04	85.67 ± 3.78
CPT-11	200	100.44 ± 0.43	108.93	9.28	107.03	8.45	86.56 ± 3.81
	1000	109.06 ± 3.82	107.74	3.90	106.8	7.14	88.61 ± 0.50
	40	114.31 ± 13.28	110.35	5.44	85.98	1.80	92.79 ± 3.15
SN-38	200	108.5 ± 16.96	86.01	4.21	84.85	4.84	90.16 ± 2.01
	1000	102.34 ± 5.21	92.94	3.38	82.51	4.15	94.59 ± 1.03
	8	99.12 ± 2.50	103.78	12.25	98.9	7.94	91.03 ± 1.66
SN-38G	40	95.08 ± 0.44	105.90	3.11	93.73	5.21	90.42 ± 2.44
	200	99.52 ± 1.49	94.36	2.48	90.88	3.15	103.7 ± 0.81