Table 3.
Extraction recovery, intra-day and inter-day precision, and matrix effects for irinotecan, SN-38 and SN-38 glucuronide in liver homogenate using MRM method (n=6)
Compound | Conc. (nM) |
Extraction recovery Average ± SD (%) |
Intra-day | Inter-day | Matrix effect Average ± SD (%) |
||
---|---|---|---|---|---|---|---|
Accuracy (bias, %) |
Precision (CV, %) |
Accuracy (bias, %) |
Precision (CV, %) |
||||
40 | 92.09 ± 8.11 | 93.87 | 2.89 | 91.01 | 0.22 | 101.01 ± 12 | |
CPT-11 | 200 | 83.94 ± 5.65 | 95.04 | 5.97 | 103.50 | 0.13 | 95.97 ± 10.9 |
1000 | 82.26 ± 4.38 | 91.55 | 2.66 | 107.77 | 0.94 | 96.3 ± 3 | |
40 | 80.28 ± 0.92 | 92.21 | 6.58 | 97.49 | 0.23 | 96.16 ± 5.4 | |
SN-38 | 200 | 80.58 ± 3.64 | 95.39 | 3.46 | 97.94 | 0.34 | 84.23 ± 3.13 |
1000 | 83.88 ± 5.98 | 96.75 | 3.74 | 97.53 | 2.19 | 95 ± 9.18 | |
8 | 111.56 ± 4.75 | 96.04 | 2.75 | 108.4 | 0.01 | 91.96 ± 10.55 | |
SN-38G | 40 | 95.51 ± 1.05 | 87.46 | 4.18 | 98.32 | 0.08 | 84.85 ± 3.95 |
200 | 84.9 ± 3.62 | 95.16 | 3.26 | 98.43 | 0.42 | 97.41 ± 0.98 |