. Author manuscript; available in PMC: 2017 Mar 15.

Published in final edited form as: J Chromatogr B Analyt Technol Biomed Life Sci. 2016 Feb 9;1015-1016:34–41. doi: 10.1016/j.jchromb.2016.02.012

Table 5.

Extraction recovery, intra-day and inter-day precision, and matrix effects for irinotecan, SN-38 and SN-38 glucuronide in feces using MRM method

Compound	Conc (nM)	Extraction recovery Average ± SD (%)	Intra-day		Inter-day		Matrix effect Average ± SD (%)

			Accuracy (bias, %)	Precision (CV, %)	Accuracy (bias, %)	Precision (CV, %)
	40	107.40 ± 8.95	90.84	2.9	81.60	1.13	94.75 ± 5.05
CPT-11	200	109.14 ± 8.45	97.22	2.33	95.86	3.53	91.86 ± 4.07
	1000	107.07 ± 1.77	97.63	0.15	81.70	2.95	85.8 ± 6.39
	40	107.02 ± 2.68	96.66	1.69	80.29	3.61	88.26 ± 2.79
SN-38	200	106.9 ± 4.51	98.97	0.94	99.43	8.88	92.31 ± 2.84
	1000	102.49 ± 2.64	93.65	2.98	89.46	4.78	115.4 ± 8.43
	8	83.8 ± 13.56	103.78	12.25	84.09	7.17	81.17 ± 4.31
SN-38G	40	93.87 ± 16.84	105.9	3.11	100.45	0.39	81.92 ± 6.08
	200	86.63 ± 16.83	94.36	2.48	106.97	9.10	97.84 ± 1.4