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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: Nat Chem Biol. 2016 Aug 29;12(10):776–778. doi: 10.1038/nchembio.2160

Figure 1. AAV-mediated incorporation of non-natural amino acids into proteins in dissociated neurons and bioorthogonal labeling.

Figure 1

(a) Non-natural amino acids used in this study. (b) Fluorescence microscopy images of untransduced cells and cells transduced as indicated to determine non-natural amino acid incorporation in dissociated rat cortical neurons. Scale bar, 200 μm. (c) Western blot analysis of non-natural amino acid-dependent production of sfGFP from sfGFP(150TAG). sfGFP incorporating 1, but not 2, was selectively labeled with 5 in an inverse electron demand Diels-Alder reaction. IP, immunoprecipitation.