Fig. 5.

ANGPT2 induces PD-L1 expression on M2-polarized monocyte-derived macrophages (MDM). A–C, MDM were differentiated from monocytes with CSF1 and then treated with ANGPT2 (300 ng/ml) for 3 days in the presence of CSF1 (A) or for 2 days in the presence of IL10 (B) or IL4 (C). MDM were sequentially stained with PE-conjugated PD-L1 antibody and FITC-conjugated CD68 antibody. Macrophages were gated on FSC/SSC and analyzed for CD68 and PD-L1 expression (A) or gated on CD68⁺ cells and analyzed for PD-L1 expression (B and C). Represntative results of at least 4 independent experiments are shown.