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. Author manuscript; available in PMC: 2017 Jan 6.

Published in final edited form as: Biochem Cell Biol. 2015 Sep 15;93(6):604–610. doi: 10.1139/bcb-2015-0007

Fig. 2 — The effect of DFF40 overexpression on DFF40, DFF45, and caspase-3 expression after incubation with doxorubicin was evaluated by real-time RT-PCR. There are 2 sets of column duplets in each diagram, one for pIRES2 empty vector and the other for pIRES2-DFF40 group. In each set of column duplet, the expression level of the genes (DFF40, DFF45, and caspase-3) was analyzed independently using the expression level of each gene in the absence of doxorubicin as the control. This notion resulted in the height of the black columns in pIRES2 empty vector or pIRES2-DFF40 vector being equal to unity. The expression of all genes was increased in DFF40 transfected cells after treatment with doxorubicin. Only caspase-3 expression level was increased in the both groups after doxorubicin treatment. *P < 0.05 and **P < 0.001 indicate statistically significant difference between two columns, illustrated using the column linking “ ”symbol.

Inline graphic — The effect of DFF40 overexpression on DFF40, DFF45, and caspase-3 expression after incubation with doxorubicin was evaluated by real-time RT-PCR. There are 2 sets of column duplets in each diagram, one for pIRES2 empty vector and the other for pIRES2-DFF40 group. In each set of column duplet, the expression level of the genes (DFF40, DFF45, and caspase-3) was analyzed independently using the expression level of each gene in the absence of doxorubicin as the control. This notion resulted in the height of the black columns in pIRES2 empty vector or pIRES2-DFF40 vector being equal to unity. The expression of all genes was increased in DFF40 transfected cells after treatment with doxorubicin. Only caspase-3 expression level was increased in the both groups after doxorubicin treatment. *P < 0.05 and **P < 0.001 indicate statistically significant difference between two columns, illustrated using the column linking “ ”symbol.