Table 1.

The role of tumor-associated macrophages (TAM) in breast cancer

Role	Identification Isolation	Tumor model	Experimental system Species	Reference
Angiogenesis	IHC: F4/80+ FC: CD11b+ F4/80+	MMTV-PyMT	Mouse, In vivo	74
	IF: F4/80+ FC: c-fms-YFP+	Met1, MVT-1	Mouse, In vivo	75
Migration	MP: Lys-GFP+, F4/80+	MMTV-PyMT MTLn3	Mouse, In vivo	79
	FC: CD11b+ Gr1- F4/80+	MMTV-PyMT	Mouse, In vivo	80
	FC: CD68+ CD206 high HLA-DR low (monocyte-derived macrophages)	MCF7, BT474, T47D, BT549, MB 436, MDA-MB-231	Human, In vitro Human, In vivo (humanized mice) Human patient samples	81
Invasion	IF/FC: CD68+ (monocyte-derived macrophages) Density gradient centrifugation	MDA-MB-231, MDA-MB-435s, BT-474, SKBR3, MCF-7	Human, In vitro Human patient samples	82
	IF: CD68+ (monocyte-deri ved macrophages)	SKBR3, MDA-MB-231	Human, In vitro	83
	Dextran+ F4/80+	MMTV-PyMT	Mouse, In vivo	84
Intravasation	MP: dextran+/Lys-GFP+/c-fms-GFP+	MMTV-PyMT	Mouse, In vivo	85
Metastasis	FC: CSF1R-eGFP+ CD11b+Gr1- F4/80+	MMTV-PyMT , Met-1, MDA-MB-231	Mouse, In vivo	90
	FC: Resident lung macrophage: F4/80+ CD11b- CD11c+ Ly6C- Metastasis-associated macrophage: CD11b+ F4/80+ CD11c- Ly6C-	MMTV-PyMT, E0771, MDAMB-231	Mouse, In vivo Human, In vivo	91
	IF: CD11b+ (together with Gr1+ or CX3CR1-GFP+ or CSF1R-GFP+)	4T1, Met-1 MDA-MB-231	Mouse, In vivo	92
	FC: CD45+ F4/80+ IF: CD68+	MDA-MB-231, CN34, 4T1	Mouse, In vivo Human, In vivo	93
Immunosuppression	FC: F4/80+ Bone marrow-derived macrophages	MMTV-PyMT	Mouse, In vivo	95