Table 2.

Twenty of the 28 genes detected in the farmed fish intestinal contents.

Classification of the antibiotics the genes confer resistance to	qPCR assay	Average relative abundance to the 16S rRNA gene
		Fish intestinal contents (sampled in 2014)	Fish farm sediments sediments (sampled in 2006–2012)
Aminoglycoside	aadA1	2 × 102	a3 × 104
Aminoglycoside	aadA2-01	2 × 102	a2 × 104
Aminoglycoside	aadA2-02	2 × 102	a4 × 104
Aminoglycoside	aadA2-03	4 × 102	a8 × 104
Trimethoprim	dfrA1	6 × 102	a3 × 103
Trimethoprim	dfrA1-02	4 × 102	b1 × 103
Class 1 integron	intI1	6 × 102	b3 × 103
Other (Antiseptic)	qacEΔ1-01	1 × 102	a5 × 103
Other (Antiseptic)	qacEΔ1-02	1 × 101	a3 × 103
Sulfonamide	sul1	7 × 102	b4 × 103
Tetracycline	tet(32)	3 × 102	a1 × 103
Tetracycline	tetM-01	1 × 101	a3 × 103
Tetracycline	tetM-02	9 × 102	a2 × 103
Tetracycline	tetM-03	4 × 102	c1 × 103
Tetracycline	tetO-01	2 × 102	a2 × 103
Tetracycline	tetW-01	4 × 102	a4 × 104
Transposon	tnpA-01	3 × 102	a6 × 104
Transposon	tnpA-04	4 × 102	a2 × 104
Transposon	tnpA-06	5 × 101	a1 × 103
Transposon	tnpA-07	4 × 102	a4 × 103

^aMuziasari et al. (2016).

^bMuziasari et al. (2014).

^cTamminen et al. (2011a).

^{a, c}The quantification of the genes using standard qPCR.

The 20 genes were the same genes found to be enriched in the sediments below fish farms in the Northern Baltic Sea, Finland. The table also shows the average relative abundance of the genes to the 16S rRNA gene in the intestinal contents and in the farm sediments. The gene assays of the qPCR array grouped by classification of the antibiotics the genes confer resistance to, class 1 integron and transposon.