Figure 2. DR6 expression on Tfh cells of lupus-prone BWF1 mice.

(a) DR6⁺ CD4⁺ cells of 24-week-old female BWF1 mice were stained with PE-conjugated anti-Bcl6 or isotype matched control Ab. The antibody specific signals of the cells are shown in histogram. (b) 7AAD⁻ CD19⁻ CD4⁺ splenocytes obtained from the indicated mice were analysed on CXCR5 versus PD1 dot plots for recognizing Tfh (PD1^high CXCR5^high) and pre-Tfh (PD1^int CXCR5^int) or non-Tfh (PD1⁻ CXCR5⁻ ) cells. (c) The Tfh cell populations of 24-week-old female BWF1 mice were sorted and then Tnfrsf21 mRNA expression in these fractionated cells was analysed by quantitative PCR (qPCR). Error bar: s.d. (n=5 per group). (d) Both DR6 and CXCR4 expressions of the indicated Tfh cell populations that were detected as in b are also shown. For detecting Tfh cell populations, gate strategy that was shown in Supplementary Fig. 15c was used in b–d.