Table 2.

mRNA expression levels in PBMCs

Gene name	Fold change (mean ± SD)			ANOVA (p value)			Type
	HV	PBC	MBC	BC specific	MBC specific	Linear
CD80	1.000 ± 0.171	2.192 ± 0.454	1.960 ± 0.187	0.039	0.308	0.066	BC-specifc
CTLA4	1.000 ± 0.162	1.651 ± 0.480	1.532 ± 0.132	0.195	0.515	0.248	Non-specific
IDO1	1.000 ± 0.140	1.935 ± 0.519	1.297 ± 0.202	0.277	0.665	0.602	Non-specific
IDO2	1.000 ± 0.449	0.777 ± 0.273	0.669 ± 0.142	0.472	0.416	0.396	Non-specific
PD1	1.000 ± 0.217	0.659 ± 0.134	0.749 ± 0.121	0.275	0.670	0.359	Non-specific
PDL1	1.000 ± 0.085	1.188 ± 0.355	2.540 ± 0.313	0.201	0.003	0.027	Linear
CD56	1.000 ± 0.214	1.481 ± 0.427	1.757 ± 0.253	0.303	0.221	0.214	Non-specific
FOXP3	1.000 ± 0.129	1.330 ± 0.298	2.944 ± 0.373	0.142	0.002	0.015	Linear
NKG2D	1.000 ± 0.131	0.768 ± 0.175	1.323 ± 0.147	0.886	0.055	0.320	Non-specific
NRP1	1.000 ± 0.287	2.185 ± 0.601	1.447 ± 0.305	0.286	0.784	0.561	Non-specific
CD4	1.000 ± 0.098	1.196 ± 0.116	1.143 ± 0.054	0.236	0.652	0.322	Non-specific
CD8	1.000 ± 0.319	0.655 ± 0.087	0.886 ± 0.073	0.245	0.668	0.567	Non-specific
CD40	1.000 ± 0.096	1.205 ± 0.122	1.424 ± 0.103	0.167	0.046	0.068	MBC-specific
CCR6	1.000 ± 0.648	1.075 ± 0.616	0.744 ± 0.116	0.874	0.464	0.658	Non-specific
CD14	1.000 ± 0.115	1.089 ± 0.186	1.680 ± 0.165	0.282	0.013	0.063	MBC-specific
CXCR4	1.000 ± 0.255	1.272 ± 0.287	1.173 ± 0.168	0.618	0.720	0.621	Non-specific

All mRNA expression levels were normalized by their mean expression levels in HVs. ANOVA with contrasts was performed to find expression patterns of the three groups (HV, PBC, MBC). The coefficients of the contrasts for group means were as follows: (−1.0, 0.5, 0.5) for BC-specific type, (−0.5, −0.5, 1.0) for MBC-specific type, and (−1.0, 0.0, 1.0) for linear type. Each hypothesis was tested at the 5 % significance level. Bold font represents significant genes by ANOVA