Table 2. Binding affinities and calculated association constants of singly and doubly phosphorylated Sic1 peptides for wild-type and allosteric site mutant Cdc4WD40.
| Sic120pS69 | Sic120pS80 | Sic120pS69/S80 | |
|---|---|---|---|
| Kd (μM) | |||
| wt Cdc4WD40 | 10.3±0.32 | 9.13±0.54 | 7.06±0.70 |
| R664Q Cdc4WD40 | 19.5±0.08 | 9.58±1.08 | 3.74±0.59 |
| Ka (M−1) | |||
| wt Cdc4WD40 | 9.76±0.31E+04 | 1.10±0.06E+05 | 1.42±0.14E+05 |
| R664Q Cdc4WD40 | 5.14±0.03E+04 | 1.04±0.12E+05 | 2.67±0.42E+05 |
Results are the average of at least three individual fluorescence-binding experiments with s.d. of all measurements reported.