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. 2017 Jan 4;8:14058. doi: 10.1038/ncomms14058

Figure 2. Downregulation of VGLL4 is associated with upregulation of Wnt target genes.

Figure 2

(a) VGLL4 mRNA levels in CRC. Levels of VGLL4 mRNA in 30 paired human CRC and normal tissues were examined by real-time PCR, using GAPDH as an internal control. The value of each bar shows the difference between the VGLL4 mRNA level in normal tissue and that in the tumour, and is represented by the equation ΔCt(N)−ΔCt(T): a value>1 indicates that the VGLL4 mRNA level is increased in the tumours, and value<−1 indicates that the VGLL4 mRNA level is decreased in the tumours. Experiments were repeated two times. Paired t test was used to compare between paired tumour and normal tissues. (b–d) mRNA levels of Wnt target genes in CRC tissue. Relative mRNA levels of Axin2 (b), CCND1 (c) and c-MYC (d) were analysed. Experiments were repeated two times. Paired t tests were used. (e) Box plots of VGLL4 and Wnt target gene levels at different tumour stages. Data are expressed as median and interquartile range. Experiments were repeated two times. Unpaired t tests were used. *P<0.05, significant relative to control. (f) Scatter plot between VGLL4 and CCND1 mRNA levels in CRC samples. VGLL4 expression was compared with CCND1 by applying Spearman's correlation. Experiments were repeated two times (Supplementary Fig. 2).