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. 2017 Jan 4;8:14058. doi: 10.1038/ncomms14058

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Copyright © 2017, The Author(s)

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(a) VGLL4 inhibited proliferation of HCT116 cells and of SW480 cells. (b) VGLL4 inhibited colony formation of HCT116 and SW480 cells. Scale bar denotes 10 mm. (c,d) Knockdown of endogenous VGLL4 by shVGLL4#1 promoted xenograft tumour growth. Mice were photographed after being killed. Black bar denotes the mean value from one experiment. Scale bar denotes 10 mm. (c) Tumours harvested from each mouse were photographed before further processing. (d) The tumour volumes were measured for the mice from each group (ten mice). (e) mRNA levels of Wnt target genes in samples from panel d. Note: Data in a,b,d and e were from one experiment. Experiments we repeated two times. Unpaired t tests were used to compare the difference between groups. *P<0.05, **P<0.01, significant relative to control. n.s., no statistics significance.