Figure 7. APC^min/+ mice were sensitive to Super-TDU treatment.

(a) Chart depicting the process used to treat APC^min/+ mice with Super-TDU. APC^min/+ and littermate wild-type (APC^+/+) mice (aged 4 w) were randomized to receive 500 μg kg⁻¹ (n=10) per day of Super-TDU peptide or control peptide (n =10) by tail vein injection. In addition, other mice were treated with 50 mg kg⁻¹ 5-fluorouracil (5-FU) intravenously as a positive control (n=10). After eight weeks, the mice (aged 12 w) were killed, and their intestines (both the small intestine and colon) were extracted. (b) Pictures of the entire small intestine show large intestinal tumours. Arrows indicate adenomas. Scale bar denotes 1 mm. (c–e) Number of tumours (c), number of tumours (length>5 mm) (d) and intestinal length (e) were calculated. Data were from one experiment. Experiments we repeated two times. Unpaired t tests were used to compare the different between groups. (f) Ki67 staining of the intestines tissue of wildtype (WT) and APC^min/+ mice treated with control, Super-TDU or 5-FU. The percentage of Ki67-positive cells were labelled below each group. Scale bar denotes 50 μm. (g) The relative expression of the Wnt target gene in intestinal tissue of wildtype (WT) and APC^min/+ mice treated with control, Super-TDU or 5-FU. (h) Survival curves of the mice. Note that all mice survived for the first 25 days of treatment, and all WT mice treated with Super-TDU, as well as those treated with the control survived for the entire treatment period. Data were from one experiment. Experiments were repeated two times. Survival curves were calculated according to the Kaplan–Meier method; survival analysis was performed using the logrank test, *P<0,05, significant relative to control. Note: Data in c–e and g were from one experiment. Experiments were repeated two times. Unpaired t tests were used to compare the difference between groups. *P<0.05, **P<0.01, ***P<0.001, significant relative to control. n.s., no statistics significance (Supplementary Figs 7 and 8).