FIGURE 8.

Effects of CdCl₂ and H₂O₂ on transient kinetics of Cd²⁺ and Ca²⁺ in roots of ectomycorrhizal (MAJ and NAU) and non-mycorrhizal (NM) Populus × canescens. Cd²⁺ (A) and Ca²⁺ (B) kinetics were recorded before and after the required amount of 50 μM CdCl₂ or 1.0 mM H₂O₂was introduced into the measuring chamber. Prior to the CdCl₂ shock, steady-state fluxes of Cd²⁺ and Ca²⁺ were monitored at the apex (measuring site was ca. 100 μm from the root tip) for approximately 10–20 min. Transient kinetics of Cd²⁺ and Ca²⁺ were recorded after the required amount of 50 μM CdCl₂ was introduced into the measuring solution. After 20–30 min continuous recording of Cd²⁺ and Ca²⁺ fluxes, Cd²⁺ and Ca²⁺ kinetics were recorded for 20 min after 1.0 mM H₂O₂ was introduced into the measuring solution. Each point represents the mean of 4–5 individual plants and bars represent the standard error of the mean.