Figure 5. Phylogenetic relationship of odorant binding proteins (OBPs) and chemosensory proteins (CSPs) and their corresponding heat maps comparing their expression levels in antennae of larvae (L), female (F) and male (M) adults of R. prolixus.

The OBP and CSP evolutionary histories were inferred using the Maximum Likelihood method in MEGA6.0. The bootstrap consensus trees (which topologies are displayed) were inferred from 1,000 pseudo-replicates. Only bootstrap values higher than 70 were displayed. Initial trees were obtained by applying the Neighbor-Joining method to a matrix of pairwise distances estimated using a LG model. Expression levels (represented as Log10 FPKM +1) were depicted with a colour scale in which white and orange represent lowest and highest expression, respectively. FX: gene model corrected based on the de novo transcriptome assembly.