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. 2017 Jan 6;7:40049. doi: 10.1038/srep40049

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Phylogenetic relationships of CheB (a) and sensory neuron membrane protein/CD36 protein (b) families and their corresponding heat maps. Heat map of ammonium transport proteins (c). Heat maps compare expression levels in antennae of larvae (L), female (F) and male (M) adults of R. prolixus. The evolutionary history of CheB and SNMP/CD36 protein families were inferred using the Maximum Likelihood method in MEGA6.0. The topologies of the CheB (highest log likelihood −1123.0068) and SNMP/CD36 trees (highest log likelihood −6147.2094) are shown. The percentage of trees in which the associated taxa clustered together is shown next to the branches. The initial trees for the heuristic search were obtained by applying the Neighbor-Joining method to a matrix of pairwise distances estimated using WAG and F models models (for CheB proteins) and L and G models (for SNMP/CD36). Expression levels (represented as Log10 FPKM +1) were depicted with a colour scale in which white and orange represent lowest and highest expression, respectively. FX: gene model corrected based on the de novo transcriptome assembly; NTE: N-terminus region is missing.