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. 2017 Jan 6;7:40023. doi: 10.1038/srep40023

Figure 7. Elimination of CHIP suppresses adipogenesis in mouse embryo fibroblast (MEF) cells.

Figure 7

(a,b) CHIP-knockout MEFs were induced to undergo accelerated adipocyte differentiation. CHIP-knockout MEFs generated through the instrumental method were induced to undergo differentiation into adipocytes through treatment with DMI and the PPARγ ligand troglitazone. (a) Differentiated cells were stained using Oil Red O and were photographed as described in Fig. 5. (b) Lipid content was analyzed by quantification kit in differentiated cells. (c,d) PPARγ protein and mRNA levels were elevated in CHIP-knockout MEFs. (c) Western blots of differentiated MEFs were performed using the indicated antibodies. (d) Messenger RNA of PPARγ and its targets was detected by qRT-PCR using the indicated primers. Data are presented means ± SD; n = 3; **P < 0.01, and ***P < 0.001 compared to each lane.