Skip to main content
. 2017 Jan 6;7:39985. doi: 10.1038/srep39985

Figure 6. NT5C interacts with the Arp2/3 complex.

Figure 6

(a) NT5C immunoprecipitates with the ARPC1B and p34 proteins. Flag immunoprecipitates from primary MEFs stably expressing Flag-NT5C and indicated mutants were probed with indicated antibodies. (b) NT5C S184A exhibits enhanced binding to ARPC1B and p34. Immunoprecipitates from NIH3T3 cells stably expressing Flag-NT5C and indicated mutants were probed with indicated antibodies. Images cropped from single gel, denoted with black line. (c) NT5C localizes to cell edge and colocalizes with p34 in primary MEFs. Cells stably expressing Flag-NT5C constructs were fixed and stained with indicated antibodies. Cells were visualized by confocal microscopy (LSM Zeiss 700). Representative images are shown. (d) NT5C localizes to cell edge and colocalizes with p34 in HT1080 cells. Cells stably expressing hNT5C#1 siRNA and Flag-NT5C constructs were fixed and stained with indicated antibodies. Cells were visualized by confocal microscopy (LSM Zeiss 700). Representative images are shown.