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. 2017 Jan 6;7:39908. doi: 10.1038/srep39908

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Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Supramolecular surface modification of viable MDAMB231 × 4 (with CXCR4-linked GFP-Tag) and MDAMB231 cells (without GFP-Tag) in mixed cell culture. Modification was accomplished via specific functionalization of the CXCR4 receptor with Ac-TZ14011-Ad, followed by host-guest interaction between β-CD molecules on fluorescent Cy5_0.5CD₁₀PIBMA₃₉ or Cy3_1.5CD₇₂PIBMA₃₈₉ polymers and the Ad functionality. Functionalization mainly occurs on the CXCR4 overexpressing MDAMB231 × 4 cells. For clarity, both the (overlay) image and the same image at the individual channels are displayed, with GFP in green, Cy5 (Cy5_0.5CD₁₀PIBMA₃₉) in red, and Cy3 (Cy3_1.5CD₇₂PIBMA₃₈₉) in blue.