Figure 3. Analysis of GC and memory B cells in immunized control and c-Abl^f/f Aicda^cre/+ mice.

Control and c-Abl^f/f Aicda^cre/+ mice were challenged with NP₃₈-CGG and analysed 10 days after immunization. (A) Flow cytometric and statistical analyses of B220⁺Fas⁺CD38⁻ GC B cells in the spleens of immunized mice. Numbers shown are percent of B220⁺ cells. (B) Flow cytometric analysis and quantification of NP-specific NIP⁺IgG1⁺ B cells in the spleens of immunized mice. Numbers shown are percent of NIP⁺IgG1⁺ B cells outside of the B220⁺Dump⁻ gate. (C) Flow cytometric analysis of NIP⁺IgG1⁺ cells to further determine CD38⁺ memory B and CD38⁻ GC B cells. Numbers indicate percent of B220⁺Dump⁻NIP⁺IgG1⁺ spleen cells analysed. Quantification of antigen specific CD38⁺IgG1⁺NIP⁺ memory B cells is shown. Each dot in the 3 graphs represent one animal analysed; ns, non-significant.