Figure 5. The relationship between autophagy and viral replication.

(a) The modification of LC3 in UV-PRV-infected Vero cells. Vero cells were mock infected or infected with UV-PRV at an MOI of 10. At 2, 4, 6, 8 and 10 hpi, the cell samples were harvested and lysed, and the cell extracts were analyzed by immunoblotting using the anti-LC3 antibody. The ratio of intensity of LC3-II to ACTB was calculated to represent the autophagic level. The data represent the mean ± SD of three independent experiments. (b) The modification of LC3 in PRV-infected Vero cells in the presence of cycloheximide (CHX). At 2 and 10 hpi, the cell samples were harvested and lysed, and the cell extracts were analyzed by immunoblotting using anti-LC3 and anti-gE antibodies. The ratio of the intensity of LC3-II to ACTB was calculated to represent the autophagic level. The data represent the mean ± SD of three independent experiments. Gels were run under the same experimental conditions. For better clarity and concise presentation, cropped blots are shown. The raw uncropped images can be found in Supplementary Fig. S3.