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. 2017 Jan 6;7:40000. doi: 10.1038/srep40000

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Copyright © 2017, The Author(s)

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(a) Specific primers for Pulsatilla species and Bgl I sites within the ITS2 region of Pu. chinensis. (b) PCR products by primers ITS2F/ITS3R and ITS2/PR1. Lane 1–22: Pu. chinensis (1–2), Pu. cernua (3–4), Pu. campanella (5–6), Pu. millefolium (7), Anemone hupehensis (8–9), Cimicifuga simplex (10–11), Clematis armandii (12–13), Potentilla chinensis (14–15), Po. discolor (16–17), Rhaponticum uniflorum (18–19), Platycodon grandiflorus (20–21) and water control (22). Lane M, DL2000 DNA marker. (c) Bgl I restriction digest pattern of PCR products of Pulsatilla species. Lane 1–7: Pu. chinensis (1–2), Pu. cernua (3–4), Pu. campanella (5–6) and Pu. millefolium (7). Lane M, DL500 DNA marker. Full-length gels are presented in Supplementary Figure S3.