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. 2017 Jan 6;7:39804. doi: 10.1038/srep39804

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Copyright © 2017, The Author(s)

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(a) Flow cytometry analysis of NeuPs and neutrophils that were sorted and cultured with G-CSF. (b) Cytological analysis of NeuPs and neutrophils cultured as in (a) and stained with May-Grünwald-Giemsa solution. (c) Experimental scheme for analysis of NeuP differentiation in vivo. NeuPs were sorted from CD45.1 congenic mice, transferred to CD45.2 recipient mice through intra-BM transfer, and analyzed in BM 3 days after transfer. (d) Expression of Ly6G (neutrophil differentiation) and CD115 (monocyte differentiation) in donor (upper three panels) and recipient cells (lower three panels), assessed by flow cytometry. Data are representative of at least two independent experiments (N = 3 mice/group).