Figure 5. NeuPs are proliferative precursor cells.
(a) Proliferation of NeuPs, neutrophils and monocytes, measured as EdU incorporation by flow cytometry. Each cell type was sorted from mice that were injected with EdU 2 hours prior to sacrifice, and stained for EdU. (b) NeuPs and neutrophils were sorted from the bone marrow of wild type mice and cultured for 2 hours in the medium supplemented with 10 μM EdU. EdU incorporation was analyzed by flow cytometry. Data are representative of two independent experiments (mean ± SD of N = 3). (c) Expression of Ki-67 of NeuPs and neutrophils, analyzed by flow cytometry. (d) Expression of c-kit on NeuPs.