Figure 1. TRIM14 negatively affects proliferation/survival of NSCLC cell lines in vitro.

(a) Schematic diagram illustrates the experimental design of the pooled RNAi screen to identify genes affecting cell proliferation/survival in H460 and H358. Deep sequencing was used to quantify TRIM14 shRNA representation in H460 and H358 cell populations at multiple time points for two independent shRNAs. (b) Profiling TRIM14 protein expression in a panel of human NSCLC cell lines of different histological subtypes via Western blot analyses. (c) Whole-cell extracts from cell lines stably expressing either full-length TRIM14 or shRNAs against TRIM14 were subjected to Western blot analysis with anti-TRIM14 antibody and compared with isogenic shGFP or empty vector (EV) controls. β-actin served as a loading control. (d) TRIM14 downregulation and overexpression in cells (red) affects cell viability as measured by MTS assays. (f) Soft agar was used to assess the ability to form colonies in isogenic cell lines. Results shown represent three biological replicates. (Two-tailed student’s t-test, ****p < 0.0001).