Table 2. Top 70 proteins that co-immunoprecipitated with TRIM14 in HEK293T cells.
| Identified Proteins (70) | Gene Symbol | Empty vector Exp. |
TRIM14 Exp. |
|||||
|---|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 1 | 2 | 3 | |||
| 1 | Tripartite motif-containing protein 14 | TRIM14 | 0 | 0 | 0 | 126 | 72 | 121 |
| 2 | DNA-dependent protein kinase catalytic subunit | PRKDC | 0 | 0 | 0 | 8 | 3 | 75 |
| 3 | Isoform 2 of E3 ubiquitin-protein ligase HUWEI | HUWE1 | 0 | 0 | 0 | 34 | 13 | 30 |
| 4 | Isoform 5 of Protein transport protein | SEC16A | 0 | 0 | 0 | 9 | 23 | 21 |
| 5 | CAD protein | CAD | 0 | 0 | 0 | 13 | 4 | 29 |
| 6 | E3 ubiquitin-protein ligase UBR5 | UBR5 | 0 | 0 | 0 | 13 | 2 | 19 |
| 7 | Isoform 2 of Melanoma-associated antigen D1 | MAGED1 | 0 | 0 | 0 | 13 | 15 | 13 |
| 8 | DnaJ homolog subfamily A member 2 | DNAJA2 | 0 | 0 | 0 | 5 | 4 | 12 |
| 9 | Melanoma-associated antigen D2 | MAGED2 | 0 | 0 | 0 | 3 | 13 | 6 |
| 10 | Translational activator GCN1 | GCN1L1 | 0 | 0 | 0 | 11 | 2 | 18 |
| 11 | Sequestosome-1 p62 | SQSTM1 | 0 | 0 | 0 | 11 | 6 | 9 |
| 12 | Bifunctional glutamate/proline--tRNA ligase | EPRS | 0 | 0 | 0 | 4 | 2 | 13 |
| 13 | Serine/threonine-protein phosphatase 6 regulatory subunit 1 | PPP6R1 | 0 | 0 | 0 | 8 | 3 | 4 |
| 14 | Isoform long of proteasome subunit alpha type-1 | PSMA1 | 0 | 0 | 0 | 9 | 4 | 5 |
| 15 | Isoform 2 of HCLS1-associated protein X-1 | HAX1 | 0 | 0 | 0 | 4 | 5 | 6 |
| 16 | Isoform 2 of Protein CIP2A | KIAA1524 | 0 | 0 | 0 | 4 | 2 | 4 |
| 17 | E3 ubiquitin-protein ligase CHIP | STUB1 | 0 | 0 | 0 | 7 | 3 | 8 |
| 18 | RuvB-like 2 | RUVBL2 | 0 | 0 | 0 | 6 | 5 | 8 |
| 19 | 26S proteasome non-ATPase regulatory subunit 4 | PSMD4 | 0 | 0 | 0 | 4 | 2 | 2 |
| 20 | E3 ubiquitin-protein ligase Praja-1 | PJA1 | 0 | 0 | 0 | 4 | 6 | 2 |
| 21 | Structural maintenance of chromosomes protein | SMC4 | 0 | 0 | 0 | 2 | 2 | 4 |
| 22 | Isoform 2 of A-kinase anchor protein 12 | AKAP12 | 0 | 0 | 0 | 3 | 3 | 2 |
| 23 | Programmed cell death protein 5 | PDCD5 | 0 | 0 | 0 | 2 | 4 | 2 |
| 24 | Nucleosome assembly protein 1-like 4 (Fragment) | NAP1L4 | 0 | 0 | 0 | 4 | 3 | 2 |
| 25 | Proteasomal ubiquitin receptor ADRM1 | ADRM1 | 0 | 0 | 0 | 4 | 0 | 2 |
| 26 | A-kinase anchor protein 8-like | AKAP8L | 0 | 0 | 0 | 19 | 8 | 0 |
| 27 | Apoptosis-inducing factor 1, mitochondrial | AIFM1 | 0 | 0 | 0 | 0 | 2 | 20 |
| 28 | DnaJ homolog subfamily C member 7 | DNAJC7 | 0 | 0 | 0 | 12 | 6 | 0 |
| 29 | Tubulin beta-8 chain | TUBB8 | 0 | 0 | 0 | 6 | 0 | 3 |
| 30 | Immunoglobulin-binding protein 1 | IGBP1 | 0 | 0 | 0 | 5 | 0 | 3 |
| 31 | Cancer/testis antigen family 45 member A5 | CT45A5 | 0 | 0 | 0 | 7 | 7 | 0 |
| 32 | Tubulin beta-2A chain | TUBB2A | 0 | 0 | 0 | 4 | 0 | 6 |
| 33 | Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 | GBF1 | 0 | 0 | 0 | 0 | 2 | 5 |
| 34 | DnaJ homolog subfamily B member 1 | DNAJB1 | 0 | 0 | 0 | 4 | 0 | 4 |
| 35 | HEAT repeat-containing protein 6 | HEATR6 | 0 | 0 | 0 | 3 | 0 | 3 |
| 36 | Structural maintenance of chromosomes protein 2 | SMC2 | 0 | 0 | 0 | 2 | 0 | 4 |
| 37 | Serine/threonine-protein phosphatase 6 regulatory subunit 3 | PPP6R3 | 0 | 0 | 0 | 3 | 0 | 4 |
| 38 | Proteasome subunit alpha type-7 | PSMA7 | 0 | 0 | 0 | 4 | 0 | 2 |
| 39 | Prefoldin subunit 2 | PFDN2 | 0 | 0 | 0 | 0 | 5 | 2 |
| 40 | La Ribonucleoprotein Domain Family Member 4B | LARP4B | 0 | 0 | 0 | 2 | 7 | 0 |
| 41 | Proteasome 26S Subunit, ATPase 6 | PSMC6 | 0 | 0 | 0 | 5 | 0 | 3 |
| 42 | Tubulin beta-4A chain | TUBB4A | 0 | 0 | 0 | 4 | 0 | 3 |
| 43 | Isoform 1 of Fanconi anemia group I protein | FANCI | 0 | 0 | 0 | 2 | 0 | 3 |
| 44 | Pyridoxal-dependent decarboxylase domain-containing protein 1 | PDXDC1 | 0 | 0 | 0 | 0 | 2 | 6 |
| 45 | Pre-mRNA-processing-splicing factor 8 | PRPF8 | 0 | 0 | 0 | 2 | 0 | 4 |
| 46 | Isoform 2 of SAM domain and HD domain-containing protein 1 | SAMHD1 | 0 | 0 | 0 | 2 | 0 | 6 |
| 47 | Pumilio homolog 1 | PUM1 | 0 | 0 | 0 | 0 | 3 | 3 |
| 48 | S-phase kinase-associated protein 1 | SKP1 | 0 | 0 | 0 | 2 | 0 | 2 |
| 49 | Isoform 2 of Cdc42 effector protein 1 | CDC42EP1 | 0 | 0 | 0 | 0 | 3 | 2 |
| 50 | Tubulin beta-6 chain | TUBB6 | 0 | 0 | 0 | 3 | 0 | 3 |
| 51 | Interleukin-1 receptor-associated kinase 1 | IRAK1 | 0 | 0 | 0 | 0 | 3 | 7 |
| 52 | Methionyl-tRNA synthetase | MARS | 0 | 0 | 0 | 2 | 0 | 5 |
| 53 | Rho guanine nucleotide exchange factor 2 | ARHGEF2 | 0 | 0 | 0 | 2 | 4 | 0 |
| 54 | BAG family molecular chaperone regulator 2 | BAG2 | 0 | 0 | 0 | 4 | 0 | 3 |
| 55 | Isoform 2 of Caseinolytic peptidase B protein homolog | CLPB | 0 | 0 | 0 | 4 | 0 | 2 |
| 56 | Acidic leucine-rich nuclear phosphoprotein 32 family member A | ANP32A | 0 | 0 | 0 | 2 | 2 | 0 |
| 57 | Reticulocalbin-1 | RCN1 | 0 | 0 | 0 | 2 | 2 | 0 |
| 58 | Cysteine and glycine-rich protein 2 | CSRP2 | 0 | 0 | 0 | 2 | 4 | 0 |
| 59 | Heat shock 70 kDa protein 4L | HSPA4L | 0 | 0 | 0 | 0 | 2 | 2 |
| 60 | Protein SEC13 homolog | SEC13 | 0 | 0 | 0 | 2 | 0 | 2 |
| 61 | RNA polymerase II-associated protein 1 | RPAP1 | 0 | 0 | 0 | 2 | 0 | 4 |
| 62 | 26S proteasome non-ATPase regulatory subunit 6 f | PSMD6 | 0 | 0 | 0 | 2 | 0 | 2 |
| 63 | Mitotic-spindle organizing protein 2A | MZT2A | 0 | 0 | 0 | 0 | 2 | 2 |
| 64 | Proteasome subunit alpha type-2 | PSMA2 | 0 | 0 | 0 | 2 | 0 | 2 |
| 65 | Isoform 2 of RING finger protein 126 | RNF126 | 0 | 0 | 0 | 2 | 0 | 2 |
| 66 | Tubulin alpha-1C chain | TUBA1C | 0 | 0 | 0 | 2 | 0 | 2 |
| 67 | DNA damage-binding protein 1 | DDB1 | 0 | 0 | 0 | 2 | 0 | 2 |
| 68 | DNA polymerase delta subunit 3 | POLD3 | 0 | 0 | 0 | 0 | 2 | 2 |
| 69 | Proteasome activator complex subunit 3 | PSME3 | 0 | 0 | 0 | 3 | 0 | 2 |
| 70 | Proteasome subunit alpha type-4 | PSMA4 | 0 | 0 | 0 | 3 | 0 | 2 |
| 71 | Mitotic spindle-associated MMXD complex subunit MIP18 | FAM96B | 0 | 0 | 0 | 2 | 0 | 2 |
Spectral counts for each of the three biological replicates are shown. Proteins in italic are involved in proteasome degradation.