Figure 3. Relaxation dispersion curves for residues in the N-terminus of the thrombin heavy chain.

Dispersion curves and data points for apo-thrombin at 800 MHz are depicted in red and 600 MHz in brown; those for PPACK-thrombin at 800 MHz are in blue and 600 MHz are in black. The dynamics of E18(39), closest to the N-terminus (blue sphere), were quenched by PPACK-binding. The dynamics of E23(44) and S27(48) were retained after PPACK-binding (red spheres).