Figure 3. Activity of I-124AAVrh.10CLN2 vector in vitro.
293ORF6 cells in a 12-well plate (4 × 105) were infected with I-124AAVrh.10CLN2 (10,000 genome copies/cell). At 72 hours post infection cells were harvested and a cell lysate was prepared (200 μl). Kinetics of TPP-1 activity were assessed with 20 μl every 10 min for 60 min using a Cytofluor 4000TC plate reader. The plate was read from the bottom using 360/20 nm excitation and 460/25 emission filters. The gain was set at 70. Uninfected cell lysate (Mock) served as the control. TPP-1 activity is expressed as mean fluorescence unit/min/mg protein ± standard error.