Table 2. The primer sequences used for real-time RT-PCR assay were listed as below.

Gene	Forward primer(5′-3′)	Reverse primer(5′-3′)
Mouse β-actin	ATGACCCAAGCCGAGAAGG	CGGCCAAGTCTTAGAGTTGTTG
Mouse TNF-α	GGAACACGTCGTGGGATAATG	GGCAGACTTTGGATGCTTCTT
Mouse IL-1β	ACCTGCTTTCCCCAAAACGAA	TGAGAGAAGTCGCACTGAGTC
Mouse HMGB-1	GGCGAGCATCCTGGCTTATC	GGCTGCTTGTCATCTGCTG
Mouse TLR-4	AGCTCCTGACCTTGGTCTTG	CGCAGGGGAACTCAATGAGG
Mouse NF-kb	ATGGCAGACGATGATCCCTAC	TGTTGACAGTGGTATTTCTGGTG
Mouse Myd88	TCATGTTCTCCATACCCTTGGT	AAACTGCGAGTGGGGTCAG
Human HMGB-1	TATGGCAAAAGCGGACAAGG	CTTCGCAACATCACCAATGGA
Human TLR4	AGACCTGTCCCTGAACCCTAT	CGATGGACTTCTAAACCAGCCA
Human Myd88	GGCTGCTCTCAACATGCGA	CTGTGTCCGCACGTTCAAGA

The primer sequences used for real-time RT-PCR.