Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jan 6;7:40191. doi: 10.1038/srep40191

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2017, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

PMC Copyright notice

NSCs derived from iPSCs in the 3D PNIPAAm-PEG hydrogel were dissociated into single cells and cultured in the 3D PNIPAAm-PEG hydrogel (a) or on Matrigel-coated 6 well plate (b–f). Cells were passaged every 5 days or 3 days for 3D and 2D cultures, respectively. (a) phase and live/dead staining images of day 5 spheroids at passage 1 (P1) and 4 (P4) in the 3D PNIPAAm-PEG hydrogels. (b) Phase images of NSCs on day 3 of passage 1, 5 and 10 (P1, P5, P10) in 2D cultures. (c) 90-95% of the NSCs at P1 and P10 in 2D cultures were positive for the NSC markers, PAX6 and NESTIN. (d) qPCR data on the expressions of pluripotency and NSC markers during P1 to P5 in 2D cultures.