Table 1. Encoding scheme for library and blocker oligonucleotides.
| Sequence (5′–3′) | a b c d | Hybridizes to | |
|---|---|---|---|
| Start | CTTGCA | ||
| Stop | TTGCAC | ||
| Bit = 0 | ATCACC | ||
| Bit = 1 | GTCTGA | ||
| Library 00 | ATCACCATCACCATCACCATCACC | 0000 | |
| Library 01 | ATCACCATCACCATCACCGTCTGA | 0001 | Blocker D0 |
| Library 02 | ATCACCATCACCGTCTGAATCACC | 0010 | |
| Library 03 | ATCACCATCACCGTCTGAGTCTGA | 0011 | |
| Library 04 | ATCACCGTCTGAATCACCATCACC | 0100 | Blocker B0 |
| Library 05 | ATCACCGTCTGAATCACCGTCTGA | 0101 | |
| Library 06 | ATCACCGTCTGAGTCTGAATCACC | 0110 | Blocker B1 |
| Library 07 | ATCACCGTCTGAGTCTGAGTCTGA | 0111 | |
| Library 08 | GTCTGAATCACCATCACCATCACC | 1000 | |
| Library 09 | GTCTGAATCACCATCACCGTCTGA | 1001 | Blocker C0 |
| Library 10 | GTCTGAATCACCGTCTGAATCACC | 1010 | Blocker A0 |
| Library 11 | GTCTGAATCACCGTCTGAGTCTGA | 1011 | Blocker A1 |
| Library 12 | GTCTGAGTCTGAATCACCATCACC | 1100 | |
| Library 13 | GTCTGAGTCTGAATCACCGTCTGA | 1101 | Blocker C1 |
| Library 14 | GTCTGAGTCTGAGTCTGAATCACC | 1110 | |
| Library 15 | GTCTGAGTCTGAGTCTGAGTCTGA | 1111 | |
| Blocker A0 | GGTGATTCAGACGGTGATTCAGAC | 1010 | Library 10 |
| Blocker A1 | TCAGACTCAGACGGTGATTCAGAC | 1011 | Library 11 |
| Blocker B0 | GGTGATGGTGATTCAGACGGTGAT | 0100 | Library 04 |
| Blocker B1 | GGTGATTCAGACTCAGACGGTGAT | 0110 | Library 06 |
| Blocker C0 | TCAGACGGTGATGGTGATTCAGAC | 1001 | Library 09 |
| Blocker C1 | TCAGACGGTGATTCAGACTCAGAC | 1101 | Library 13 |
| Blocker D0 | TCAGACGGTGATGGTGATGGTGAT | 0001 | Library 01 |