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. 2017 Jan 6;7:39823. doi: 10.1038/srep39823

Figure 2. Knockdown of Rad51 with siRNA stimulated Netrin-1 induced axonal branching.

Figure 2

(A–D) Representative images of E14.5 cortical neurons transduced with siRNA oligonucleotides targeting Rad51 (Rad51 siRNA) or scramble control (Scram siRNA) and labelled with TuJ1 antibody. (E–H) Higher magnification images of boxed areas in (A–D). Scale bars are 10 μm. (I–K) Bar graphs showing comparison of branching dynamics in Vehicle (PBS), scrambled control siRNA, or Rad51siRNA-treated neurons following application of PBS or Netrin-1 in relation to (I) axon length (μm), (J) neurite number, or (K) filopodia per 20 μm of axon. Values are mean ± SEM, numbers of neurons analysed are depicted at base of corresponding bars, *p < 0.05, **p < 0.001, ***p < 0.0001.