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. 2017 Jan 6;7:39823. doi: 10.1038/srep39823

Figure 3. Overexpression of Rad51 abolished axonal branching in response to Netrin-1.

Figure 3

Representative images of TuJ1-immunostained axons (red) treated with PBS (A–C) or Netrin-1 (D–F) following transfection with EYFP fused to an empty plasmid (Empty, A,D), wildtype RAD51 (wtRAD51, B,E), or the RAD51 R250Q mutant (R250Q, C,F). Scale bar is 5 μm. Note that images with TuJ1 and EYFP co-staining are presented in Supplementary Fig. S4. (G) Bar graph showing quantification of Netrin-1 induced neuritogenesis from neurons expressing the different constructs, and (H) as in (G) but showing quantification of filopodia number per 20 μm of axon. Values are mean ± SEM, *p < 0.05, **p < 0.001, ***p < 0.0001.