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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Insect Mol Biol. 2016 Nov 7;26(1):113–126. doi: 10.1111/imb.12275

Figure 5.

Figure 5

Detection of AeMMP1 and AeMMP2 antigens by Western blot analysis using pAb generated against peptides derived from each AeMMP. (A) Western blot detection of AeMMP1 antigen by pAb-mmp1 and (B) AeMMP2 antigen by pAb-mmp2 in midguts of mosquitoes that had received a sugarmeal, a bloodmeal diluted 1:1 with non-infected cell culture medium, or a bloodmeal diluted 1:1 with CHIKV-infected cell culture medium (titre in the bloodmeal: 107 pfu/ml) at 24, 48, and 72 h post-feeding (BF). β-actin was used as a loading control. Molecular masses in kDa are indicated. Data in the figure are representative of experiments that have been repeated twice.