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. 2017 Jan 6;7:40360. doi: 10.1038/srep40360

Figure 2. TNTs form between infected and uninfected A549 cells.

Figure 2

(a, panel i and ii) TNTs readily form between uninfected A549 cells labelled with membrane dye DiD (red), or between A549 cells infected with PR8 virus and labelled with DiO (Green). After 1 h of co-culture nanotubes formed by uninfected and infected A549 cells were assessed (Scale bars: 10 μm). (a, panel iii and iv) TNTs were also seen readily connecting neighboring A549 cells either uninfected (red) or PR8 infected (green) cells. Corresponding bright field images are also shown. (b and c) Panel showing increased frequency of TNT formation in A549 cells infected with PR8 virus when compared to uninfected cells. The frequency of TNT formation between uninfected and PR8 infected A549 cells is shown after 2–4 h of incubation at 37 °C (n = 500; number of cell counts over three independent experiments); difference between uninfected and infected cells is significant (t-test, P < 0.05).