TABLE 2.
Mapping T-cell epitopes on A. centrale OMP7 and A. marginale OMPs 7 to 9
| Peptide | Sequencea | T-cell proliferation (SI)b |
|---|---|---|
| A. marginale OM | 26.0 (1.3) | |
| A. centrale OMP7 peptide 8, A. marginale OMP 7 to 9 peptide 7 | ||
| 7C_7 | LEAVRERFPIWKVGGRTWTKGDSLFLLVDD | 1.4 (0.8) |
| 7C_8 | GDSLFLLVDDAIVRLATGQSDHDDPAAKAL | 15.4 (4.5) |
| 7C_8.1 | SLFLLVDDAIVR | 20.7 (1.0) |
| 7C_8.2 | LFLLVDDAIVR | 15.7 (8.4) |
| 7C/7M_8.3 | FLLVDDAIVR | 13.7 (8.0) |
| 7C_8.4 | LLVDDAIVR | 0.7 (0.1) |
| 7C_8.5 | FLLVDDAIV | 33.6 (0.2) |
| 8/9M_7.1 | FLLVDDAVVR | 26.2 (6.9) |
| A. centrale OMP7 peptide 14 | ||
| 7C_14 | KLSFIEAALSRIGGHKIEIPAVVANTFGAN | 14.9 (2.1) |
| 7C_14.1 | KLSFIEAALSRIG | 11.8 (4.0) |
| 7C_14.2 | LSFIEAALSRIG | 28.4 (5.4) |
| 7C_14.3 | SFIEAALSRIG | 9.3 (5.0) |
| 7C_14.4 | GHKIEIPAVVANT | 0.5 (0.1) |
| 7M_14.4 | GYKIEIPAVAANT | 1.2 (1.1) |
| 8M_14.4 | GHKIEIPAVAANT | 0.6 (0.1) |
| 9M_14.4 | GYRIKIPAVVANT | 0.3 (0.1) |
| Similar sequences | ||
| AM1137 | VLSYIEAAGLRIV | 22.3 (1.9) |
| AM171 | SLAFILAVLSV | 2.1 (2.7) |
| AM1313 | KSFIELAVLSK | 0.8 (0.9) |
a
Overlapping 10-amino-acid peptide sequences are underlined.
b
A 2-week-old T-cell line from animal 48422 (DRB3*1201/*1201) was used. Standard deviations are indicated in parentheses. Responses were considered significant if the stimulation index (SI) was >4.0 (indicated by boldfaced type).