IgG cross-reactivity against recombinant dimeric OspC proteins from different B. burgdorferi strains. (A) Immunoblot analysis of murine sera. Sera from D-OspCB31-immunized (left panel) (pool of sera from two animals) or M-OspCB31-immunized (right panel) (pool of sera from two animals) mice were tested for reactivity to different Borrelia strains. N40, 107
Borrelia burgdorferi N40; 297, 107
Borrelia burgdorferi 297; B31, 107
Borrelia burgdorferi B31, M, molecular mass (in kilodaltons) standards; D-OspCB31, 50 ng recombinant D-OspCB31; M-OspC297, 50 ng recombinant M-OspCB31. (B) Limited IgG cross-reactivity against recombinant dimeric OspC proteins from different B. burgdorferi strains by ELISA. Sera from immunized animals were tested against the indicated antigen: alum only (white, pool of sera from 9 animals), D-OspCB31 (black, pool of sera from 3 animals), D-OspC297 (shaded, pool of sera from 12 animals), and D-OspCN40 (dotted, pool of sera from 12 animals). Bars represent mean ± standard deviation (SD) absorbance.