FIG 6.
Murine sera from protected D-OspCB31-immunized animals inhibited binding of monoclonal antibodies protective against B. burgdorferi B31 challenge. Sera from mice immunized with alum only, D-OspCB31, or M-OspCB31 were used in ELISA to compete for binding with biotinylated monoclonal antibodies 1-5A10 and 2-2H8, and the proportion of inhibition was plotted. The antigen on the ELISA plates was D-OspCB31. Sera were from mice immunized with alum only (white), M-OspCB31 (gray), or D-OspCB31 (black). Clone 1-5A10 was protective against B. burgdorferi challenge. Clone 2-2H8 reacted with the loop 5 epitope and was not protective. Each group consisted of 12 animals. Bars represent the mean ± SD proportion of inhibition.